About me

苟士学中国科学院广州生物医药与健康研究院助理研究员
电子邮件: gou_shixue@gibh.ac.cn
地址：广州市萝岗区开源大道190号

Education

2012-2016 河南师范大学生物技术学士
2016-2018 中国科学院广州生物医药与健康研究院发育生物学硕士
2018-2021 中国科学院广州生物医药与健康研究院发育生物学博士
2021-2023 中国科学院广州生物医药与健康研究院发育生物学博士后
2023至今中国科学院广州生物医药与健康研究院助理研究员

Science and Research

NGS data analysis (RNA-seq, ChIP-seq, ATAC-seq, CUT&Tag, WES, WGS, scRNA-seq and scATAC-seq)
Public database data mining: TCGA and GEO
Gene editing, Gene therapy and Animal model of human disease (pig, rabbit, mouse, rat)
early embryonic development (ZGA initiation)

Programming skills

Shell, R, Python, Docker, Git
Workflow & environment management: Shell, Snakemake, Conda
Website development & interactive visualization: RShiny, CSS, HTML

Published papers

Li N, Gou S, Wang J, Zhang Q, Huang X, Xie J, Li L, Jin Q, Ouyang Z, Chen F, Ge W, Shi H, Liang Y, Zhuang Z, Zhao X, Lian M, Ye Y, Quan L, Wu H, Lai L, Wang K. CRISPR/Cas9-Mediated Gene Correction in Newborn Rabbits with Hereditary Tyrosinemia Type I. Mol Ther. 2021 Mar 3;29(3):1001-1015. doi: 10.1016/j.ymthe.2020.11.023. Epub 2020 Nov 20. PMID: 33221434; PMCID: PMC7934638.
Chen M, Shi H, Gou S, Wang X, Li L, Jin Q, Wu H, Zhang H, Li Y, Wang L, Li H, Lin J, Guo W, Jiang Z, Yang X, Xu A, Zhu Y, Zhang C, Lai L, Li X. In vivo genome editing in mouse restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers. Genome Med. 2021 Apr 12;13(1):57. doi: 10.1186/s13073-021-00876-0. PMID: 33845891; PMCID: PMC8042958.
Jin Q, Yang X, Gou S, Liu X, Zhuang Z, Liang Y, Shi H, Huang J, Wu H, Zhao Y, Ouyang Z, Zhang Q, Liu Z, Chen F, Ge W, Xie J, Li N, Lai C, Zhao X, Wang J, Lian M, Li L, Quan L, Ye Y, Lai L, Wang K. Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Sci China Life Sci. 2022 Nov;65(11):2269-2286. doi: 10.1007/s11427-021-2088-1. Epub 2022 May 18. PMID: 35596888.
Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L. Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors. Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8. PMID: 36323848; PMCID: PMC9630288.
Xie J, Huang X, Wang X, Gou S, Liang Y, Chen F, Li N, Ouyang Z, Zhang Q, Ge W, Jin Q, Shi H, Zhuang Z, Zhao X, Lian M, Wang J, Ye Y, Quan L, Wu H, Wang K, Lai L. ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems. BMC Biol. 2020 Sep 23;18(1):131. doi: 10.1186/s12915-020-00866-5. PMID: 32967664; PMCID: PMC7510086.
Jin Q, Liu X, Zhuang Z, Huang J, Gou S, Shi H, Zhao Y, Ouyang Z, Liu Z, Li L, Mao J, Ge W, Chen F, Yu M, Guan Y, Ye Y, Tang C, Huang R, Wang K, Lai L. Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation. Genome Biol. 2023 Jan 17;24(1):8. doi: 10.1186/s13059-023-02851-x. PMID: 36650523; PMCID: PMC9843877.
Liang Y, Xie J, Zhang Q, Wang X, Gou S, Lin L, Chen T, Ge W, Zhuang Z, Lian M, Chen F, Li N, Ouyang Z, Lai C, Liu X, Li L, Ye Y, Wu H, Wang K, Lai L. AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns. Nucleic Acids Res. 2022 May 20;50(9):5384-5399. doi: 10.1093/nar/gkac353. PMID: 35544322; PMCID: PMC9122597.
Wang X, Liang Y, Zhao J, Li Y, Gou S, Zheng M, Zhou J, Zhang Q, Mi J, Lai L. Generation of permanent neonatal diabetes mellitus dogs with glucokinase point mutations through base editing. Cell Discov. 2021 Oct 12;7(1):92. doi: 10.1038/s41421-021-00304-y. Erratum in: Cell Discov. 2021 Dec 9;7(1):120. PMID: 34635647; PMCID: PMC8505425.
Zhou J, Liu Y, Wei Y, Zheng S, Gou S, Chen T, Yang Y, Lan T, Chen M, Liao Y, Zhang Q, Tang C, Liu Y, Wu Y, Peng X, Gao M, Wang J, Zhang K, Lai L, Zou Q. Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Mol Ther. 2022 Jul 6;30(7):2443-2451. doi: 10.1016/j.ymthe.2022.04.010. Epub 2022 Apr 20. PMID: 35443934; PMCID: PMC9263286.
Li Y, Wang H, Chen H, Liao Y, Gou S, Yan Q, Zhuang Z, Li H, Wang J, Suo Y, Lan T, Liu Y, Zhao Y, Zou Q, Nie T, Hui X, Lai L, Wu D, Fan N. Generation of a genetically modified pig model with CREBRF^R457Q variant. FASEB J. 2022 Nov;36(11):e22611. doi: 10.1096/fj.202201117. PMID: 36250915.
Wang X, Shi H, Zhou J, Zou Q, Zhang Q, Gou S, Chen P, Mou L, Fan N, Suo Y, Ouyang Z, Lai C, Yan Q, Lai L. Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation. J Genet Genomics. 2020 May 20;47(5):249-261. doi: 10.1016/j.jgg.2020.05.002. Epub 2020 May 19. PMID: 32703661.
Lian M, Chen F, Huang X, Zhao X, Gou S, Li N, Jin Q, Shi H, Liang Y, Xie J, Ge W, Zhuang Z, Wang J, Ye Y, Yang Y, Wang K, Lai L, Wu H. Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants. J Genet Genomics. 2021 Jan 20;48(1):92-95. doi: 10.1016/j.jgg.2020.07.010. Epub 2020 Nov 7. PMID: 33504469.
Xie J, Ge W, Li N, Liu Q, Chen F, Yang X, Huang X, Ouyang Z, Zhang Q, Zhao Y, Liu Z, Gou S, Wu H, Lai C, Fan N, Jin Q, Shi H, Liang Y, Lan T, Quan L, Li X, Wang K, Lai L. Efficient base editing for multiple genes and loci in pigs using base editors. Nat Commun. 2019 Jun 28;10(1):2852. doi: 10.1038/s41467-019-10421-8. PMID: 31253764; PMCID: PMC6599043.
Jiang Z, Qin L, Tang Y, Liao R, Shi J, He B, Li S, Zheng D, Cui Y, Wu Q, Long Y, Yao Y, Wei Z, Hong Q, Wu Y, Mai Y, Gou S, Li X, Weinkove R, Norton S, Luo W, Feng W, Zhou H, Liu Q, Chen J, Lai L, Chen X, Pei D, Graf T, Liu X, Li Y, Liu P, Zhang Z, Li P. Human induced-T-to-natural killer cells have potent anti-tumour activities. Biomark Res. 2022 Mar 24;10(1):13. doi: 10.1186/s40364-022-00358-4. PMID: 35331335; PMCID: PMC8943975.